2.7. Qualitative analysis by HPLC
For HPLC analysis, 1 g of freeze dried powdered plant material
was extracted with MeOH:H2O (1:1, 1 20 ml) for 2 h at room
temperature followed by hydrolysis with 1 N HCl by refluxing on
a water bath. The hydrolysate was filtered and fractionated with
ethyl acetate (EtOAc, 2 10 ml).