Methods: Eighty ovariectomized adult rats were allocated to five groups: group 1, sham-operated (SHAM); group 2–5, ovariectomized (OVX). Semipurified diets containing 0.7% calcium and 0.5% phosphorus were fed for 16weeks, group 1 and group 2 got no supplements, group 3 (PRO) was supplemented with a potential probiotic(Lactobacillus acidophilus NCC90), group 4 (PRE)with prebiotics (oligofructose+acacia gum) and group 5 (SYN)with synbiotics (probiotics + prebiotics).