MATERIAL AND METHODS
Milking of the animals
For milking, 10 Holstein cows (age range, 3–7 years; average
weight, 580 kg; average milk production, 25 L day−1; and lactation
period, 100–150 days after calving), located at the dairy house of
the Federal University of Vic¸osa, Brazil, were randomly selected for
the study. The experiment was conducted in randomized blocks,
with each cow representing one block.
MATERIAL AND METHODSMilking of the animalsFor milking, 10 Holstein cows (age range, 3–7 years; averageweight, 580 kg; average milk production, 25 L day−1; and lactationperiod, 100–150 days after calving), located at the dairy house ofthe Federal University of Vic¸osa, Brazil, were randomly selected forthe study. The experiment was conducted in randomized blocks,with each cow representing one block.
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