Isolation of viruses of thermophilic Archaea—Sulfolobus are
often not the dominant organisms in hot springs with temperature
>70°C and pH <4 (Snyder et al. 2004). Furthermore,
Sulfolobus virus sequence diversity decreases with enrichment
(Snyder et al. 2004). Therefore it is likely that the viruses
reported to date are considerably fewer and less diverse than
the viruses present in situ. Direct TEM imaging of concentrated
samples from both acidic and neutral hot springs indicate
that a number of viruses with novel morphology remain
to be isolated. (Rice et al. 2001; Rachel et al. 2002).
Comparison of methods for isolation of haloviruses and viruses
of thermophilic Archaea—The main difference between the two
main techniques described here is due to the relative abundance
of viruses and VLPs in the environments of the hosts.
There are many more viruses in hypersaline environments
than in thermoacidophilic ones. Therefore direct isolation
has been successful for haloviruses, but not for viruses of ther-
การแปล กรุณารอสักครู่..
