The induced shoot buds proliferated on 10 M Kn, 4.5 M
BAP and 5.5 M NAA supplemented medium (Fig. 1E). Individual
(0.3–0.5 cm) shoots were separated from the clump of proliferated
shoots and transferred to elongation medium containing different
concentrations and combinations of plant growth regulators
(PGRs) such as BAP, IAA, NAA and IBA (Table 3). Significant differences
in elongation were observed at different concentrations and
combinations of PGRs and genotypes. A combination of BAP and
IAA was found to be best for all the genotypes. The best elongation
(3.01–3.61 cm) was observed on a medium containing 2.25 M
BAP and 8.5 M IAA (Fig. 1F). The amount of elongation ranged
from 1.91 to 3.61 cm on media containing combinations of BAP
and IAA. Elongation was inhibited on medium containing BAP and
IBA. The amount of elongation ranged from 2.01 to 2.31 cm on