The aim of the present work was to develop a new fully validated
HPLC/ESI-MS method for the simultaneous determination
of iridoids, secoiridoids and xanthones in G. lutea samples that
were cultivated for the first time in the Monti Sibillini National
Park (Italy), collected wild in the March-Umbria area, or bought
in herbal shops, and to evaluate the quality of the G. lutea L.
cultivated in the Park. To our knowledge, this is the first time that
HPLC/ESI-MS was used for simultaneous quantification of these
compounds in G. lutea.