The appropriate selection of
temperature values in the treatment of smoothies with high hydrostatic pressure permits cold
pasteurization at pressure levels well below 300 MPa to be obtained, thus enabling cost reduction to
manufacturing plants and making the industrialization of the process favourable. Selective and recovery
media counts indicated that the content of injured L. monocytogenes LGB cells in the survivor population
reached a maximum value after the most severe treatments. Regarding such a cell fraction, microscopy
observations of LIVE/DEAD BacLight and JC-1 staining appear to indicate a widespread presence of
plasmolysis in cells with a partially depolarized membrane, providing additional information about their
physiological state.
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