2.3 Solid Fermentation of Monascus purpureus
The dried rice were placed in several jars (300 g, each) and autoclaved for 15 minutes. These jars were cooled and the dried rice was inoculated with 10% of Monascus culture. Solid fermentation of Monascus strain was carried out at 30oC, for 14 days, these substrates was supplemented with 2 mL of YMP medium for five days and mixed for everyday. Monascus fermented rice (MFR) product was dried at 50oC for overnight. Extraction of MFR product was carried out by percolation in ethanol 95% with ratio 1:10 by using a column for overnight and filtrate was evaporated by a vacuum evaporator. MFR extract was then kept in a refrigerator.