Growth in various salt concentrations
(0–20% NaCl), at different pH values (4–11) and at
various temperatures (4–60 uC) were tested in nutrient
broth. All tests were carried out by incubating the cultures
at 37 uC, except for investigations into the effect of
temperature upon growth. Acid production profiles from
carbohydrates were obtained with the API 50 CH system
(bioMe´rieux) after growth in 50 CHB medium as described
by Logan & Berkeley (1984)