such as carbon substrates, is probably a principal factor that affects the persistence of E. coli O157:H7 in natural environments, such as soil and water [6]. Utilization of organic materials (e.g., farmyard manure and slurry) is the most economic and practical option for improving soil quality while also providing additional sources of nutrients for growing plants [8]. Approximately 1011 ton of agricultural animal manure are produced globally each year and routinely applied to the soil as a crop fertilizer [11]. However, the application of organic materials could increase the incidence of disease caused by soil-borne fungal pathogens [12,13]