glucose
reacted faster than lactose, being the monosaccharide ten-fold
reactive than the disaccharide. Even at 24 h of storage, significant
differences (P < 0.05) between lysine contents in both systems
were detected. The difference between both rates was explained
by the increase in the reactivity of the reducing sugar with the proportion
of the open chain form but also by the effect of steric hindrance
caused by the size of the sugars (Chevalier, Chobert, Mollé,
& Haertlé, 2001). As carbohydrates do not interfere, this method
can be used even in systems with high levels of sugars, such as
dairy products.