2.4. Analysis of antioxidant extracts
2.4.1. 1,1-Diphenyl-2-picrilhydrazil (DPPH)
The capacity for capturing free radicals was determined using the radical 1,1-diphenyl-2-picrilhydrazil (DPPH) (Brand-Williams, Cuvelier, & Berset, 1995). To 0.1 mL of a concentration of 1 mg/L from the sample in methanol, 2.9 mL of the methanol solution of DPPH (36 mg/mL) were added and the mixture was shaken vigorously. The vials remained at ambient temperature for 30 min, before measuring absorbance at 517 nm with a UVeVIS