Fig. 4.
Symbiotic seed germination and protocorm developmental stages of D. officinale using mycorrhizal fungi Tulasnella sp., JC-02, cultured on oat meal agar 11 weeks after sowing; (a) stage 0; (b) arrow show stage 1 and star show stage 2; (c) arrow show stage 3 and star show stage 4; (d) stage 5 of protocorm developments; note elongation of first leaf. e peloton structures and coiled hyphae in the cortical cells of JC02+ protomeristem stained with 0.05% (w/v) trypanblue in lactoglycerol; (f) D. officinale plants after 49 days of inoculation, note: the JC02+ plants (left) and the control (right). Bar: (a) 0.25 mm, (b) 0.5 mm, (c) 0.25 mm, (d) 1 mm, (e) 20 μm and (f) 2 cm.