1. Place 1 mL of ACD or 3 mL of Alsever's solution into a sterile bottle with a lid.
2. Bleed the first chicken and take 1 mL of blood. Immediately remove the needle from the syringe, gently push down the plunger and transfer the blood to the bottle. Replace the lid on the bottle and rotate it gently to mix.
3. Repeat Step 2 and take 1 mL of blood from a second chicken. Transfer the blood to the bottle of blood and anticoagulant and rotate it gently to mix.
4. Repeat Step 2 and take 1 mL of blood from a third chicken. Transfer the blood to the bottle of blood and anticoagulant and rotate it gently to mix.
The bottle now contains either 3 mL of blood mixed with 1 mL of ACD OR 3 mL of blood mixed with 3 mL of Alsever's solution.