In Experiment 2, the explanatory variable that was included in the statistical model was the occurrence of estrus. Dependent variables (i.e., diameter of the LF at device removal, diameter of the LF at TAI, CL diam-eter 7 d after TAI, and serum P4 concentrations 7 d after TAI — only for those females that had ovulated after the TAI synchronization protocol) were analyzed by a two-way ANOVA using PROC GLM. Response variables were tested according to their homogeneity and normality of variances using Guide Data Analysis from SAS. The square root transformation of P4 con-centrations were subjected to ANOVA. Data are pre-sented as meanSD.