In conclusion, cultivation of oyster mushroom on various agricultural residues offers economic initiatives for agribusiness to examine these residues as valuable resources and use them to produce protein rich mushroom products. In this study, highly prized edible mushroom, namely Pleurotus sajor-caju was tested to grow on different agricultural residues such as banana leavepea residue and maize stalk as base materials with rice bran and chicken manure as supplements. Nepal as an agricultural country, large quantity of these residues has been produced. Maize stalk was the best and cheap alternative substrate for the cultivation of oyster mushroom (P. sajor-caju) with rice bran supplement followed by pea residue and banana leaves. The addition of rice bran with different substrates resulted in an increase in yield and biological efficiency. The study revealed that pea residue can also be used as alternative substrate in the cultivation of P. sajor-caju.