3.2. In-house enzyme production and activity
The conversion of enzymatic biomass-to-ethanol processes are affected by the use of purchased hydrolysis enzyme preparations. Cellulase cost is estimated at a minimum of $10/kg protein [24]. Accordingly, it is necessary to reduce the cost and amount of enzymes required for biomass hydrolysis to economical process. Various enzymatic compositions produced and secreted by fungi, and it has very different effects on biomass depolymerization. Here, we produced a suitable enzyme complex for okara hydrolysis using okara waste as the carbon source.