Then 1.0 mL of a sodium carbonate saturated solution was added, and
followed by the addition of Nanopure water filled to volume. Solutions
were mixed and allowed to stand at room temperature for
2 h. Sample aliquots were filtered through a Whatman 0.45 m poly
(tetrafluoroethylene) filter prior to the determination of total phenolics
concentration using a UV spectrophotometer monitoring
640 nm.