Substances present in tobacco form a complex mixture
of organic and inorganic compounds that may interact
to produce additive, synergistic or antagonistic effects
(Fent, 2003). In the present study, the results of the Comet
assay and MN test (Table 1 and Figure 1) of hemolymph
from snails (H. aspersa) fed tobacco leaves were significantly
higher than those of the control group (fed lettuce).
The application of the Comet assay in these snails has been
shown to be an inexpensive, effective and sensitive method
for assessing the genotoxicity of chemical mixtures
(Ianistcki et al., 2009).