Stock solutions of each aflatoxin
(AFB
1
, AFG
1
, AFB
2
and AFG
2
) were prepared according to the Man-ual of Official Methods of Analysis (AOAC, 1995 ) in benzene-acetonitrile and a mixed solution obtained containing each
aflatoxin: AFB
1 and AFG
1
at 5lg/mL and AFB
2 and AFG
2
at
1.5 l g/mL