2DEwithIPGs isoneof themost powerful tools for large-scale
protein separation and quantification [1]. In 2DE, proteins
are separated according to pI in the first-dimensional IEF
and are then separated according to molecular weight (Mr)
in the second-dimensional SDS-PAGE [2]. Currently, 2DE
combined with MS is the major platform for proteomics [3].
The aim of most 2DE analyses is to maximize the number