The combined liquefaction stream and yeast propagation tank
were consistently contaminated and are likely sources of persistent
contamination in this biofuel facility. The most abundant contaminants
identified were Lactobacillus sp., particularly L. plantarum,
L. casei, L. mucosae, and L. fermentum. Biofilm-forming strains were
present throughout the survey and may be involved in the persistence
of contamination. However, there was no apparent correlation
between the ability of isolates to form biofilms and the
capacity to inhibit ethanol production. Ethanol inhibition appeared
to be related to acetic acid production, particularly by obligately
heterofermentative species such as L. fermentum and L. mucosae
The combined liquefaction stream and yeast propagation tankwere consistently contaminated and are likely sources of persistentcontamination in this biofuel facility. The most abundant contaminantsidentified were Lactobacillus sp., particularly L. plantarum,L. casei, L. mucosae, and L. fermentum. Biofilm-forming strains werepresent throughout the survey and may be involved in the persistenceof contamination. However, there was no apparent correlationbetween the ability of isolates to form biofilms and thecapacity to inhibit ethanol production. Ethanol inhibition appearedto be related to acetic acid production, particularly by obligatelyheterofermentative species such as L. fermentum and L. mucosae
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