Twenty-nine strains isolated from sugarcane molasses and
grapes were assayed for their ability to produce ethanol. Yeast
samples were aseptically collected from local sugarcane mills
(Tucum an, Argentina) and vineyards located in Salta (Argentina),
plated individually on YPS agar supplemented with antibiotics
(ampicillin 20 g/L, tetracycline 10 g/L, chloramphenicol 20 g/L, and
eritromicin 20 g/L) to suppress bacterial contaminants[10], serially
diluted, plated and growth at 30
C for 24 h. Isolated colonies were
dispensed into fresh medium containing 40% (v/v) glycerol as
cryoprotectant, and maintained at20
C for further assays.