. From Fig. 1, the growth index of H. callitrichoides was affected by the concentration of the nutrients in the liquid MS media. The mean growth index of explant cultured in full strength MS, 1/2 strength MS, 1/4 strength MS and 1/8 strength MS were 7.34 _ 0.17 g/g, 5.52 _ 0.4 g/g, 5.20 _ 0.47 g/g and 4.48 _0.28 g/g respectively.