E.coli OD600, wet weight, dry weight, cell volume, cell number and cell density
OD stands for optical density. The number following the OD indicates the wavelength of light. OD600, OD550, OD650 or other wave lengths may be used to estimate the E.coli cell density. OD600 is the most commonly used. OD600 measures the light absorbance of an E.coli cell culture sample. The OD600 value corresponds with the cell density or cell number in a given E.coli culture volume. Different cell strains may have different cell numbers at a given OD600 value, but OD600 = 1 usually means there are about 1 x 109 cells per ml culture. Different growth conditions will also give different OD600 values.
OD600 is used to measure the E.coli cell density. The OD600 value is also an important indicator of the physiological condition of the E.coli cells in a given medium after a specific culture period. The OD600 value determines if the E.coli cells may be ready for making competent cells, cell stocks, induction, or for being harvested.
OD measurement is more accurate at values less than 0.5 for most spectrometers. For common medium this means 10 time dilution of the culture. For high density growth medium, this means 100 time dilution. Distilled or deionized water should be used as a blank and in dilution. Rich media often have color by themselves. OD measurement will not be accurate if a medium is used as a blank or in dilution.
E.coli OD600, wet weight, dry weight, cell volume, cell number and cell density
OD stands for optical density. The number following the OD indicates the wavelength of light. OD600, OD550, OD650 or other wave lengths may be used to estimate the E.coli cell density. OD600 is the most commonly used. OD600 measures the light absorbance of an E.coli cell culture sample. The OD600 value corresponds with the cell density or cell number in a given E.coli culture volume. Different cell strains may have different cell numbers at a given OD600 value, but OD600 = 1 usually means there are about 1 x 109 cells per ml culture. Different growth conditions will also give different OD600 values.
OD600 is used to measure the E.coli cell density. The OD600 value is also an important indicator of the physiological condition of the E.coli cells in a given medium after a specific culture period. The OD600 value determines if the E.coli cells may be ready for making competent cells, cell stocks, induction, or for being harvested.
OD measurement is more accurate at values less than 0.5 for most spectrometers. For common medium this means 10 time dilution of the culture. For high density growth medium, this means 100 time dilution. Distilled or deionized water should be used as a blank and in dilution. Rich media often have color by themselves. OD measurement will not be accurate if a medium is used as a blank or in dilution.
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