In zero-exchange superintensive culture systems, flocculated particles (bioflocs) accumulate in the water
column. Consequently, some control over the concentration of these particles must be performed. The
objective of this study is to evaluate the effects of three concentrations of bioflocs on microbial activity,
selected water quality indicators and performance of Litopenaeus vannamei in a tank system operated
with no water exchange. A 44-day study was conducted with juvenile (6.8 g) shrimp stocked in twelve
850 L tanks at a stocking density of 459 shrimp m−3. Biofloc levels were expressed as three presets of total
suspended solids (TSS) concentrations, as follows: 200 mg L−1 (T200), 400–600 mg L−1 (T400–600), and
800–1000 mg L−1 (T800–1000). TSS levels were controlled by attaching a 40 L settling tank to each culture
tank. Reduction of TSS to concentrations close to 200 mg L−1 decreased the time of bacterial cell residence
and significantly reduced the nitrification rates in the water (P < 0.05). The tanks in the T200 treatment
had a greater variability of ammonia and nitrite (P < 0.05), which led to the need to increase the C:N ratio of
the organic substrate to control ammonia through its assimilation into heterotrophic bacterial biomass.
But the higher production of heterotrophic bacteria in T200 (P < 0.05) increased the dissolved oxygen
demand. Nitrification rates were higher (P < 0.05) in tanks with TSS concentrations above 400 mg L−1,
and ammonia and nitrite were significantly lower than in the T200 tanks. We suggest that ammonia and
nitrite in the T400–600 and T800–1000 tanks were controlled primarily by nitrifying bacteria, which
provided higher stability of these parameters and of dissolved oxygen. Regarding shrimp performance,
the reduction of TSS to levels close to 200 mg L−1 was associated with better nutritional quality of bioflocs.
Nevertheless, differences in biofloc levels and nutritional quality were not sufficient to affect the weight
gain by shrimp. The rate of shrimp survival and the final shrimp biomass were lower (P < 0.05) when the
TSS concentrations were higher than 800 mg L−1. Analysis of the shrimps’ gills showed a higher degree of
occlusion in the T800–1000 treatment (P < 0.05), which suggests that the shrimp have an intolerance to
environments with a solids concentration above 800 mg L−1. Our results show that intermediate levels
of bioflocs (TSS between 400 and 600 mg L−1) appear to be more suitable to superintensive culture of L.
vannamei since they create factors propitious for maintaining the system’s productivity and stability