The ovarian tissue was cut into blocks (<1mm3) and fixed by phosphate buffer and 2.5%glutaraldehyde for 2 h.A0.1 mmol/L phosphoric acid bleaching lotion was used to rinse the blocks for 15 min, repeat three times prior to be dehydrated by various concentrations of ethanol and acetone. Samples were then sliced into 50e60nmby using an LKB-I ultra-thin microtome and then negative-stained by uranyl acetate and lead citrate. A JEM-1200-ex transmission electron microscope (JEOL Ltd., Tokyo,Japan) was used in this experiment.
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