The experimentwas run in a set of 10 flasks, each carries 100mg
of PCL film piece in 90 mL MSM and inoculated with 10 mL (v/v) of
24 h old culture. The flasks were incubated in an orbital shaker
incubator at 50 C and 150 rpm for 10 days. Both biotic (without PCL
film piece) and abiotic (without bacterial cells) controls were run in
parallel with test. The experiment was run in triplicate.