Two species of Red Sea puffer fishes L. sceleratus Fig. 1 and A.
hypselogenion Fig. 2, were chosen for the present study. The
crude bioactive materials from their ovaries and skin mucus
were isolated and their antifouling property was tested. The
samples were collected from the Red Sea fishing ports and were
taken to the laboratory of NIOF (National Institute of Oceanography
and Fisheries) in Suez for immediate processing. The
ovaries of L. sceleratus were homogenized with 1% acetic acid.
The obtained homogenate was centrifuged at 2700 rm. Then
the pellet re-extracted twice with the same volume of 1% acetic
acid solution. The supernatant was concentrated under
reduced pressure and then defatted by shaking gently with
an equal volume of dichloromethane three times. The aqueous
layer was collected and concentrated under reduced pressure to
remove residual dichloromethane. The resulting crude extracts
were stored in a freezer till later testing.