For the exclusion of outliers in replicates, itwas used the Grubbs test
using Microsoft Excel® (Microsoft, Version 2010) software. The
GraphPad InStat (Version 3.00, 1997) softwarewas used for application
of t-Student test (comparison of two averages) and Tukey–Kramer test
(comparison of three or more averages). A 95% significance level was
adopted for all comparisons.