Dates Syrup containing 174.0 ± 0.2 kg m3 and
358.0 ± 1.0 kg m3 was supplemented with mineral culture
medium as described previously by Djelal et al. [25]. The pH
was adjusted to 6.0 using KOH 1000 molm3
. The medium was
transferred into 500 cm3 bottles with a final working volume of
300 cm3, which were autoclaved at 120 C for 20 min before
adding the NH4Cl sterilized by filtration on a 0.2 mmmembrane
(Sartorius, Goettingen, Germany).