The working area of a screen-printed electrode, SPE, was modified with the enzyme tyrosinase (Tyr) using different immobilization methods, namelyentrapment with water-soluble polyvinyl alcohol(PVA), cross-linking using glutaraldehyde(GA), and cross-linking using GA and human serum albumin(HSA); the resulting electrodes were termed SPE/Tyr/PVA, SPE/Tyr/GA and SPE/Tyr/HSA/GA, respectively. These biosensors were characterized by means of amperometry and EIS techniques. From amperometric evaluations, the apparent Michaelis-Menten constant,Km′, of each biosensor was evaluated while the respective charge transfer resistance, Rct, was assessed from impedance measurements. It was found that the SPE/Tyr/GA had the smallest Km′(57 ± 7) μMand Rct values. This electrode also displayed both the lowest detection and quantification limits