Another observation was the particle aggregation at high tem-perature over time for vesicles without Salmonella’s presence,improved system size (from 300 nm to 700 nm) and zeta poten-tial (from −30 to −20). After Salmonella’s addition, the interactionbetween the bacteria and the antibody stabilized PDA/antibody
vesicles over time keeping the particle size and the zeta poten-tial constant; while incubation temperature influenced mainly Salmonella’s development improving zeta potential. Once bothSalmonella and the antibody have negative superficial charge, theirinteraction was via affinity rather than electrostatic interaction