Enriched culture (100 lL) was surface plated on the solidified
Dorn’s medium supplemented with fipronil, in 90 mm Petri dishes
followed by incubation at 28 C. After 7 d, when well isolated bacterial
colonies appeared, individual colonies were picked up with a
sterile needle loop and streak purified on the same medium. The
growing individual colonies from the streak plate were ultimately
maintained by growth in slant tubes prepared with the fipronil
supplemented Dorn’s medium as well as on LB agar medium and
maintained at 4 C.