In this study, we introduce quantum dots (QDs) as fluorescent probes and immunochromatographic
strips to develop quantitative fluorescence point-of-care tests (QF-POCT)
to analyze C-reactive protein (CRP) levels. Goat anti-rabbit IgG and rabbit IgG were used as
control antibodies, and mouse monoclonal CRP antibody pairs were used for disease marker
detection. One monoclonal CRP antibody was conjugated with QDs and served as a signal antibody,
and the other monoclonal CRP antibody was dispensed onto the nitrocellulose membrane
and served as a capturing antibody. In the presence of CRP, the fluorescence intensity of the
monoclonal antibody-CRP-monoclonal antibody sandwich complex captured on the nitrocellulose
membrane was determined using the fluorescence strip reader.