(A)–(I) Different stages of guayule transformation using callus. (A) Leaf pieces placed on CIM facing abaxial surface for callus induction. (B) Growth of calli on CPM. (C) Co-cultivation of calli with Agrobacterium on CCM. ((D)–(F)) Kanamycin selection of Agrobacterium infected calli on CPM, SIM and SPM, respectively. (G) Selection of transformed shoots on SEM. (H) Rooting of transformed shoots on RIM. (I) Hardening of transgenic plant in greenhouse