Clostridium thermocellum 27405 (from David Levin, Univ. of Manitoba) was maintained at 55 C by routinely transferring 10% (v/v) inocula into fresh ATCC 1191 medium [23], supplemented with avicel cellulose (0.5%, w/v) as the sole carbon substrate
Clostridium thermocellum 27405 (from David Levin, Univ. of Manitoba) was maintained at 55 C by routinely transferring 10% (v/v) inocula into fresh ATCC 1191 medium [23], supplemented with avicel cellulose (0.5%, w/v) as the sole carbon substrate