After being subjected to the antimicrobial bioassay described
above, twenty-five termite whole guts from each treatment were
dissected, pooled, and homogenized in 750 mL of sodium phosphate
buffer (0.05 M, pH 7.0). The homogenate was centrifuged for 15 min
at 14,000 rcf at 4 C and the supernatant collected for use in assays
described below. This procedure was repeated across treatments for
each of three termite colonies which served as biological replicates.