Since the initial commercialization of Flow Cytometry (FC) and Fluorescence Activated Cell Sorting (FACS) in 1968, they have undergone significant improvement. However, there remain numerous impediments, other than cost, to the further acceptance of the technology by many laboratories. Technical issues persist around detection of low abundance molecules in intracellular compartments, the lack of "universal" cell permeabilizing chemistries, confounding effects from cell autofluorescence, overlap of emission spectra between fluorochromes, and unavailability of reagents for targeting molecules of interest. Specifically for cell sorters, there are problems of cell survival after pressure changes during droplet formation and collection, dilution of the sorted cells prior to reanalysis or culture, and the time delay it takes to obtain sufficient number of viable cells. Lastly, data analysis is tricky, particularly when dealing with low abundance targets.