Loopful of
24 hours old bacterial cultures were inoculated into 30
mL BH medium with 1 % xylene and ethereal solution
of phenanthrene, 0.5 % ethereal solution of
naphthalene, biphenyl and 0.5 % anthracene solution
(dissolved in hexane) and left the flasks opened near
the flame in hood to allow evaporation of organic
solvents and were incubated 35 o
C in an orbital shaker
operated at 150 rpm for 8-16 days.