Interest in trehalase was boosted when very potent and highly specific competitive inhibitors of trehalase were discovered and applied to insects. These inhibitors, such as trehazolin ()or validoxylamine A (), are analogues of trehalose of bacterial origin that bind tightly to trehalase. when injected into insects, these inhibitors affect motor activity, feeding , metabolism, growth, development, reproduction and flight (), thus indicating that trehalose metabolism is involved in many aspects of insect physiology. The inhibitors interfere specifically with the hydrolysis of trehalose () but apparently do not block its production and release into the haemolymph by the fat body.()The prominent inhibitor-induced increase in haemolymph trehalose was noticed early and has been reported repeatedly.()The precipitous decrease in haemolymph glucose was later discovered as a spectacular metabolic effect that appeared to be a crucial factor in trehazolin toxicity in locusts.()