Agar diffusion was carried out with RPMI 1640 agar supplemented with glucose 2%. The inoculum was prepared using 24-hour plate cultures of Candida and Cryptococcus species. The colonies were suspended in 0.85% saline and the turbidity was compared with the 0.5 McFarland standard, to produce a yeast suspension of 1x106 to 5x106 cells/mL. The suspension was loaded on a sterile cotton swab that was rotated several times and press firmly against the