After filtration,washing and work up, 40g ofthe resultant extract (“crude ginger extract”) were applied to a silica gel column and sequentially eluted with solvents of increasing polarity to yield fractions 1 through 11, which were chemically characterized by HPLC (Fig.1C) and/or GC–MS and screened invitro for their ability to inhibit PGE2 production from an LPS-stimulated human macrophage cell line, as previously described.