release glucose since most of high potential lactic acid bacteria lack of amylolytic enzymes. Enzymatic starch hydrolysis usually involves at least two enzymes; α-amylase (EC 3.2.1.1) and amyloglucosidase or glucoamylase (EC 3.2.1.3) [9, 10] and overall process requires at least 48-72 h. The incorporation of starch hydrolysis as a part of lactic acid production process causes obvious drawbacks of being time and cost-consuming as well as the inhibition of enzymes involved in starch hydrolysis due to the production of high glucose concentration (11). The mentioned obstacles can be overcome by an operation of simultaneous saccharification and fermentation (SSF) process, which is a process that combines the steps of saccharification by glucoamylase in starch hydrolysis and fermentation by microbial starters (3, 5). With SSF, the saccharification and fermentation take place simultaneously and the released glucose will be gradually converted into lactic acid.