The complexes and the ligand were screened for their in vitro
antibacterial activity against Pseudomonas aeruginosa (Gram-negative),
Escherichia coli (Gram-negative) and Staphylococcus aureus
(Gram-positive) and the antifungal activity against Candida albigans
(Gram-positive). The tests were performed using the disc diffusion
method [15] using nutrient broth medium [containing (g/L):
beef extract 3 g; peptone 5 g; pH 7.0]. The sterile paper discs
(0.5 mm) impregnated with compound dissolved in dimethylsulfoxide
(DMSO) at a concentration of 100 lg/mL were used. Then,
the paper discs impregnated with the solution of the compound
tested were placed on the surface of the media inoculated with
the microorganism. The plates were incubated at 35 C for 24 h.
Reading of the results was done by measuring the diameters of
the inhibition zones generated by the tested substances using a ruler.
Amikacin and ketoconazole were used as positive control for
bacteria and fungus respectively.