The sample or standard in
aliquots of 50 ll was mixed with 50 ll diluted FC reagent (1:5, v/
v in water) followed by addition of 100 ll 0.7 M NaOH. The
absorbance was measured at 760 nm using a BioTek Synergy 2
multi-code micro plate reader (BioTek, Vermont, USA) and the total
phenolic content (TP) was expressed as mg of gallic acid equivalents
(GAE) per 100 g of dry matter (DM).