Each method requires a different number of steps for the final β-glucan preparation. More steps imply more equipment, materials, time and a raised production cost. Production of β-glucans with Methods 1 and 7 is achieved only in three steps, but method 7 requires yeast cell walls as a starting material.[21, 27] Methods 2 and 8 are completed in four steps but Method 8 needs also prepared yeast cell walls.[22, 28] The other methods require more steps in which the lipids and proteins are removed from the yeast cells with the use of organic solvents and enzymes or DEAE chromatography, leading to more purified fractions of β-glucans.[23-26] The steps needed for each different β-glucan extraction method are named and reviewed in Table 1.