Molecular taxonomy of the isolates
To identify ITS sequences similar to those obtained from fungi
isolates, the FASTA algorithms were used to screen the EMBL/Genbank database of fungal nucleotide sequences. To visualize the
diverse fungal taxa identified by means of molecular characters,
a sequence similarity dendrogram was developed with the ITS1-
5.8S rRNA-ITS2 nucleotide sequences of the isolates. Isolates
sequences were aligned using the program ClustalX 2.1 with the
default settings, and the dendrogram produced with MEGA 6.06
software using the neighbor-joining method with Kimura 2-
parameter distances. Groups of sequences at close proximity
within the same branch of the dendrogram were individually
aligned with ClustalX to determine their percentage of similarity.
Sequences with similarity greater than 99% were considered