2.10. Identification of LAB isolates
The genus and species were confirmed using molecular identification.
Bacterial DNA from each strain was extracted using a
PureLink Genomic DNA Mini Kit (Invitrogen). PCR reactions were
carried out in a final volume of 50 ml containing 25 ml of TopTaq
Master Mix (Qiagen), 1 ml of each primer (27f/1512r), 2 ml of DNA
and 21 ml of RNase free water. The PCR products were purified with
Purelink PCR Purification Kit (Invitrogen). The purified PCR products
were sequenced by Macrogen Inc. (Seoul, South Korea) using
an ABI3730 XL automatic DNA sequencer. Sequences were then
compared to those in the GenBank database using the BLAST