Protein extracts containing 100 ng of total protein were separated on a 12.5% (w/v) acrylamide gel (Laemmli, 1970) along with 200 ng of commercially available lactoferrin (Sigma) as a standard.
Protein extracts containing100 ng of total protein were separated on a 12.5% (w/v)acrylamide gel (Laemmli, 1970) along with 200 ng of commerciallyavailable lactoferrin (Sigma) as a standard.